大鼠丙氨酸轉(zhuǎn)氨酶(ALT)elisa檢測試劑盒 ，英文名：Rat ALT ELISA Kit
產(chǎn)品簡單說明：丙氨酸轉(zhuǎn)氨酶(ALT)是由活性細(xì)胞產(chǎn)生并能在體內(nèi)外起催化作用的一種特殊蛋白質(zhì)。轉(zhuǎn)氨酶在氨基酸的合成與分解代謝中起重要作用，種類很多，不同轉(zhuǎn)氨作用由不同的轉(zhuǎn)氨酶所催化, 谷丙轉(zhuǎn)氨酶存在于肝、心、骨骼肌和腎等臟器的細(xì)胞內(nèi)，肝細(xì)胞里面zui多。
更多大鼠ELIS試劑盒產(chǎn)品！
ELISA的比色測定由酶標(biāo)儀進(jìn)行。有的同行可能會認(rèn)為，既然由酶標(biāo)儀進(jìn)行，那么此時便可以萬事大吉了，其實(shí)不然，因?yàn)楫?dāng)代較為先進(jìn)的酶標(biāo)儀，均有較多的功能，使用不當(dāng)，會得到令人難以理解的大鼠丙氨酸轉(zhuǎn)氨酶(ALT)elisa檢測試劑盒實(shí)驗(yàn)結(jié)果。如使用酶標(biāo)儀比色測定后，有許多的陰性測定孑L的吸光度值為負(fù)數(shù)；或測定假陽性率大大增加等等。這主要是沒有正確地理解和使用酶標(biāo)儀所致。
此外，在加入底物開始顯色反應(yīng)前，是先檢查一下底物溶液的有效性，即可將A和B兩種液各加1滴于清潔的空板孔或eppendorf管中，觀察是否有顯色出現(xiàn)，如有，則說明底物已變質(zhì)。以O(shè)PD為底物，配好后應(yīng)為五色，否則就不能使用。以TMB為底物，整個顯色反應(yīng)過程無需避光，而以O(shè)PD為底物，則需避光進(jìn)行。關(guān)于TMB和OPD的顯色反應(yīng)特點(diǎn)及注意點(diǎn)可參考前面有關(guān)章節(jié)內(nèi)容。顯色反應(yīng)完成后，加入酸終止反應(yīng)，振蕩混勻后即可進(jìn)行下面的比色測定或肉眼判斷結(jié)果。21792 紫草Y122594 "牛七堿 " Szechenyine "≥98% "
Y122595 牛磺雄去氧膽酸 Aesculetin "≥98% "
Y122596 牛蒡子苷元 Arctigenin "≥98% "
Y122597 牛蒡子苷 Arctiin "≥98% "
Y122598 "檸檬酸 " Citric acid monohydrate "≥98% "
Y122599 "尿囊素 " Allantoin "≥98% "
Y122600 "鳥苷 " GUANOSINE "≥98% "
Y122601 擬人參皂苷-RT5 Pseudoginsenoside-RT5 ≥98%
Y122602 牡荊素葡萄糖苷 Vitexin-4 "≥95% "
Y122603 牡荊素 Vitexin "≥98% "
Y122604 莫諾苷 （莫諾忍冬苷） Morroniside 97%
Y122605 "棉子糖 " Raffinose "≥98% "
Y122606 "棉籽糖 " Raffinose "≥98% "
Y122607 迷迭香酸 Rosmarinic acid "≥97% "
Y122608 霉酚酸酯 Mycophenolate mofetil "≥98% "
Y122609 沒食子兒茶素沒食子酸酯 (-)-Gallocatechin gallate "≥98% "
Y122610 沒食子兒茶素 Gallocatechin (GC) 97%
Y122611 毛蕊異黃酮-7-O-β-D葡萄糖苷 calycosin-7-O-β-D-glucoside 97%
Y122612 毛蕊異黃酮 Calycosin "≥98% "
Y122613 毛蕊花糖苷/類葉升麻苷/麥角甾苷 Verbascoside "≥98% "
Y122614 莽草酸 Shikimic acid "≥98% "
Y122615 "芒柄花素-7-O-葡萄糖苷 " "Formononetin-7-O-β-D-glucoside " "≥98% "
Y122616 芒柄花素-7-O-β-D-葡萄糖苷 Fomononetin-7-O-glucoside ≥95%
Y122617 "麥冬皂苷D " Ophiopogonin D ≥97%
Y122618 麥冬皂苷B Ophiopogonin B ≥97%
Y122619 馬兜鈴酸B Aristolochic acid B "≥98% "
Y122620 馬兜鈴內(nèi)酰胺 Aristololactam "≥98% "
Y122621 麻醉椒苦素 Methysticin "≥99% "
Y122622 "落新婦苷 " Astilbin ≥98%
Y122623 "洛緦 " "Rosin " "≥95% "
Y122624 洛塞維 Rosavin "≥95% "
Y122625 "洛塞琳 " "Rosarin " "≥95% "
Y122626 羅漢果皂苷IV Mogroside IV "≥98% "
Y122627 羅漢果甜苷VI Mogroside VI "≥98% "
Y122628 羅漢果甜苷V Mogroside V "≥98% "
Y122629 氯化木蘭花堿 Magnoflorine chloride "≥98% "
Y122630 蘆竹堿 Gramine "≥98% "
Y122631 蘆丁 Rutin "≥95% "
Y122632 遼東楤木皂苷X "3-O-{[β-D-glucopyranosyl(1→2)]-[ β-D-glucopyran
osyl(1→3)-β-D-glucopyranosyl(1→3)] β-D-glucopyranosy
l}hederagenin 28-O-β-D-glucopyranosyl ester" 95%
Y122633 遼東楤木皂苷V 3-O-[β-D-glucopyranosyl(1→2)]-[ β-D-glucopyranosyl(1→3)-β-D-glucopyranosyl(1→3)] β-D-glucopyranosyl hederagenin 95%
Y122634 連翹脂素 Forsythiaside "≥97% "
Y122635 連翹脂苷 Forsythiaside ≥95%
Y122636 "櫟櫻酸 " Roburic acid ≥98%
Y122637 利血平 Reserpine "≥98% "
Y122638 雷公藤甲素 Triptolide "≥98% "
Y122639 雷公藤紅素 Celastrol "≥98% "
Y122640 萊鮑迪苷 Rebaudioside-A 98%
Y122641 辣椒堿 Capsaicin "≥98% "
Y122642 奎寧 Quinine "≥95% "
Y122643 苦玄參苷IA Picfeltarraenin IA "≥98% "
Y122644 苦鬼臼毒素 Picropodophyllotoxin "≥98% "
Y122645 柯諾辛 Corynoxine "≥94% "
Y122646 開環(huán)異落葉松樹脂酚二葡萄糖苷 Secoisolariciresinol diglucoside ≥98%
Y122647 "開環(huán)異落葉松樹脂酚 " Secoisolariciresinol "≥98% "
Y122648 桔皮素 Tangeretin "≥98% "
Y122649 桔梗皂苷D PlatycodinD "≥98% "
Y122650 酒石酸長春質(zhì)堿 Catharanthine Tartrat ≥98%
Y122651 酒石酸長春瑞濱 Vinorelbine tartrate "≥97% "
Y122652 京尼平苷 Geniposide "≥98% "
Y122653 金絲桃素 Hypericin "≥95% "
Y122654 絞股藍(lán) A Fiveleaf Gynostemma Herb "≥98% "
Y122655 "假荊芥屬苷 " Nepetin-7-glucoside "≥97% "
Y122656 "甲殼素 " Chitin "≥98% "
Y122657 甲基蓮心堿大鼠丙氨酸轉(zhuǎn)氨酶(ALT)elisa檢測試劑盒 Neferine 98%
Y122658 吉馬酮 Germacrone "≥98% "
Y122659 積雪草酸 Asiatic acid ≥97%
在目前的以HRP作為標(biāo)記酶的商品市場ELISA試劑盒中，如以TMB為底物，則提供的底物為A和B兩瓶應(yīng)用液；如以O(shè)PD為底物，則試劑盒提供OPD片劑或粉劑，臨用前配制。一般商品大鼠丙氨酸轉(zhuǎn)氨酶(ALT)elisa檢測試劑盒顯色反應(yīng)條件為37℃或室溫反應(yīng)15～30min。從理論上說，37℃30min才可以使HRP的底物催化反應(yīng)*，盡管在zui初的10min內(nèi)，絕大部分催化反應(yīng)即可完成。因此，為使弱陽性樣本孔能有充分的顯色，建議在37℃下反應(yīng)25～30min后，終止反應(yīng)比色測定。
