Gelatin Verona Buffer (GVB) and Verona Buffered Saline (VBS) are essential buffers for complement research. Compared to Gelatin Verona Buffer (GVB), Verona Buffered Saline (VBS) lacks gelatin components.

Aimijie Gelatin Flora Buffer without Ca++and Mg++:

Cat B103-250mL;  B101-1L

Name GVBo (GVB without Ca++and Mg++) Gelatin Flora Buffer without Ca++and Mg++

Ingredients: 0.1% gelatin, 5mM Vernal, 145mM NaCl, 0.025% NaN3, pH7.3

Apply GVBo to prepare rabbit red blood cells (Er) and clean them before use for assay; GVBo can be used to dilute all components of the complement bypass pathway test.

Concept:

During the dissolution process, two types of changes occur: one is the diffusion of solute molecules (or ions) into water under the action of water molecules, which absorbs heat; the other is the interaction between solute molecules (or ions) and water molecules, generating water and molecules (or water and ions), which release heat.

There are two changes during the dissolution process of substances:

Diffusion process: The diffusion of solute molecules (or ions) into water is a physical process that absorbs heat

Hydration process: the molecules (or ions) of solute interact with water molecules to generate hydrated molecules (or hydrated ions), which is a chemical process and emits heat

Temperature changes during dissolution process:

a. The heat absorbed during the diffusion process is greater than the heat released during the hydration process, resulting in a decrease in solution temperature, such as NH4NO3 dissolved in water.

b. The heat absorbed during the diffusion process is less than the heat released during the hydration process, and the solution temperature increases, such as NaOH or concentrated sulfuric acid dissolved in water.

c. The heat absorbed during the diffusion process ≈ the heat released during the hydration process, and the temperature of the solution remains almost constant, such as NaCl dissolved in water.

明膠弗洛拿緩沖液(Gelatin Veronal Buffer, GVB)和弗洛拿緩沖的生理鹽水(Veronal Buffered Saline, VBS)是補體研究的必-備緩沖液, 弗洛拿緩沖的生理鹽水(VBS)和明膠弗洛拿緩沖液(GVB)相比缺少明膠組分。   

艾美捷不含Ca++和Mg++的明膠弗洛拿緩沖液：

Cat B103-250mL; B101-1L

名稱 GVBo (GVB without Ca++ and Mg++)不含Ca++和Mg++的明膠弗洛拿緩沖液

成分 0.1% gelatin, 5mM Veronal, 145mM NaCl, 0.025% NaN3 , pH7.3.

應用 GVBo應用于制備兔紅細胞(Er)，并在用于測定前對其進行清洗; GVBo可用于稀釋補體旁路途徑試驗的所有組分。

概念：

在溶解過程中發(fā)生了兩種變化一種是溶質的分子（或離子）在水分子的作用下向水中擴散這一過程吸收熱量另一種是擴散的溶質的分子（或離子）和水分子作用，生成水和分子（或水和離子），這一過程放出熱量。

物質溶解過程中的兩種變化：

擴散過程：溶質的分子（或離子）向水中擴散，是物理過程，吸收熱量

水合過程：溶質的分子（或離子）和水分子作用，生成水合分子（或水合離子），是化學過程，放出熱量

溶解過程中的溫度變化：

a.擴散過程中吸收的熱量>水合過程中放出的熱量，溶液溫度降低，如：NH4NO3溶解于水。

b.擴散過程中吸收的熱量<水合過程中放出的熱攝，溶液溫度升高，如：NaOH、濃硫酸溶解于水。

c.擴散過程中吸收的熱量≈水合過程中放出的熱量溶液溫度幾乎不變，如：NaCl溶解于水。