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上海邦景實(shí)業(yè)有限公司
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閱讀:340發(fā)布時(shí)間:2014-2-17
Supplies:
Modified Eagle’s Minimum Essential Medium
·Earle’s Balanced Salt Solution (BSS)
·2 mM L-glutamine
·0.1 mM nonessential amino acids
·1.5 g/L sodium bicarbonate
·1.0 mM sodium pyruvate
·20% Fetal Bovine Serum (FBS)
·1% Penicillin-Streptomycin (pen/strep) antibiotic solution
Method: ( to be done approximay every 2 days)
1.Heat media to 37 degrees Celsius in water bath.
2.In laminar flow hood, aspirate media with glass Pasteur pipet connected to house vacuum line.
3.Add 15 mL of media.
4.Tightly cap and return to 37 degrees Celsius incubator.
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