Anti-Brdu ,FITC conjugated
Catalog Number : Rs-0489R-FITC
Quantitysize : 0.1ml (2mg/ml; Reconstitute with 0.25 mlof sterile distilled water.)
Theimmunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNAis a powerful tool to study the cytokinetics of normal and neoplastic cells. Invitro or in vivo labeling of tumor cells with the thymidine analogue BrdU andthe subsequent detection of incorporated BrdU with specific anti-BrdUmonoclonal antibodies is an accurate and comprehensive method to quantitate thedegree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA ofS-phase cells may provide an estimate for the fraction of cells in S-phase.Also dynamic proliferative information such as the S-phase transit rate and thepotential doubling time can be obtained, by means of bivariate BrdU/DNA flowcytometric analysis
pecificity :
· Anti-Brdu isa rabbit polyclonal antibody, FITC conjugated
specificfo Brdu of Human, Mouse, Rat, Dog, Horse
· use for westernblotting,elisa,immunoprecipitation and immunohistochemistry
· Protein G affinity chromatographypurification, purity :>95%
· Isotype: IgG
· mol wt: 48 kDa
Application :
· FCM: 1:20-100
· Immunohistochemistry : 1:20-200
· Immunocytochemistry: 1:20-200
· Excitation spectrum: 490-495nm
· Emission spectrum: 520-530nm
· Optimal working dilutions must bedetermined by the end user.
Storage: Store at -20℃ for one year. Avoid repeated freeze/thawcycles. The lyophilized antibody isstable at room temperature for at least one month and for greater than a year when kept at -20℃. Whenreconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for atleast six weeks at 2-4 ℃
ImportantNote: This product as supplied is intended for research useonly, not for use in human, therapeuticor diagnostic applications.
