Anti-NCOA5抗體操作說明書 antibody
FROM:RICKY
Catalog Number :RS-86178R
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ） background:
Nuclear receptors for steroids, thyroid hormones and retinoic acids are liganddependent transcription factors that activate transcription through specific DNA binding sites in their target genes. NCoA-5 (nuclear receptor coactivator 5), also known as CIA (coactivator independent of AF-2), is a widely expressed nuclear receptor coregulator consisting of 579 amino acids. NCoA-5
acts as a coactivator for ERα (estrogen receptor α), ERβ (estrogen receptor β) and Rev-erbβ (orphan nuclear hormone receptor BD73), and corepresses c-Myc in response to 17-β-estradiol. NCoA-5 contains one LxxLL (Leu-Xaa-Xaa-Leu-Leu) motif that allows it to successfully associate with nuclear receptors,and the NCoA-5 gene maps to human chromosome 20q13.12.
Specificity :
–Anti-NCOA5 is a rabbit polyclonal antibody
–specific for anti-NCOA5 of rat ,mo ,hu
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
–Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 66 kDa
Application :
- WB=1:100-500
- ELISA=1:500-1000
- IHC-P=1:100-500
- IHC-F=1:100-500
– not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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