Anti--CD30/TNFRSF8/FITC
Catalog Number : RSF-2495R-FITC
Quantity size : 100ug （2mg/ml,Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 10mg/ml BSA and 0.1% Sodium azide. ）
Background: CD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkin's and Reed-Sternberg cells using monoclonal antibody Ki-1. The ligand for CD30 is CD30L （CD153）. The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas （including Burkitt's lymphomas）, virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that coexpress CD45RO and the IL4 receptor..
Specificity : · Anti- -CD30/TNFRSF8 is a rat monoclonal antibody, /FITC
· specific for -CD30/TNFRSF8 of hu
· Protein G affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt: 63kDa
Application : Flow-Cyt=1:50-200
IF=1:50-200
· Immunohistochemistry : 1:20-1:200
· Immunocytochemistry: 1:20-1:200
· Excitation spectrum: 490-495nm
· Emission spectrum: 520-530nm
. KLH conjugated synthetic peptide derived from mouse TNFRSF
· Optimal working dilutions must be determined by the end user.
· protect from light.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
